淋巴系统是免疫系统的重要部分，与血管系统共同作用来保持组织的内环境稳定。淋巴系统从组织中得到液体和大分子物质并将其返回血液循环，因此，淋巴系统在调节组织中液体，蛋白质和压力平衡方面起着重要作用。尽管淋巴毛细管内皮细胞与血管内皮有许多相似的特性，但由于结构不同，又各具特点。淋巴毛细管缺少壁细胞且以基底膜不完整或缺失为特征。淋巴内皮的特征是包含大量的内褶和细胞质囊泡，并且具有交错的细胞间连接。淋巴毛细管*显著的特征是与间质的一体化，间质通过细微的弹性纤维与细胞外基质相连。
淋巴内皮细胞提取于人淋巴结组织，**代冻存。每管含有细胞数>5×10^5cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基：内皮细胞培养基(ECM, Cat. #1001)
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货号
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2500
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产地
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美国
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缩写
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HLEC
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规格
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5 x 10^5 /1ml
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用途
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科研
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储存
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液氮
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运输
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干冰
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关键字
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人淋巴内皮细胞 HLEC 人淋巴内皮细胞 HLEC 人淋巴内皮细胞 HLEC 人淋巴内皮细胞 HLEC 人淋巴内皮细胞 HLEC 人淋巴内皮细胞 HLEC
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公司简介
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合肥星肽生物科技有限公司作为专业的ScienCell中国代理商，专业经营实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务，对购买项目的前期资料提供,中期合同保证,后期货物跟踪到*终售后的确保项目准确到位，都有相关专业人士进行维护,确保您在合肥合肥星肽生物科技有限公司公司获得*上等服务！
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联系方式
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何经理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898，
E-mail：info@qingbio.com http://www.qingbio.com QQ:514713116
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1.) Speyer CL, Hachem AH, Assi AA, Johnson JS, DeVries JA, Gorski DH. (2014) 'Metabotropic Glutamate Receptor-1 as a Novel Target for the Antiangiogenic Treatment of Breast Cancer.'
2.) Speyer CL, Hachem AH, Assi AA, Johnson JS, DeVries JA, Gorski DH. (2014) "Metabotropic Glutamate Receptor-1 as a Novel Target for the Antiangiogenic Treatment of Breast Cancer."?PloS one. 9: e88830.
3.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-? in human cholangiocarcinoma QBC939 cells promote tumor progression." PLoS One. 8: e62024.
4.) Park HS, Jung IM, Choi GH, Hahn S, Yoo YS, Lee T. (2013) 'Modification of a Rodent Hindlimb Model of Secondary Lymphedema: Surgical Radicality versus Radiotherapeutic Ablation.'
5.) Park HS, Jung IM, Choi GH, Hahn S, Yoo YS, Lee T. (2013) "Modification of a Rodent Hindlimb Model of Secondary Lymphedema: Surgical Radicality versus Radiotherapeutic Ablation."?BioMed res int. 2013: 208912.
6.) Qiu Y, Chen Y, Fu X, Zhang L, Tian J, Hao Q. (2012) "HMGB1 promotes lymphangiogenesis of human lymphatic endothelial cells in vitro."?Med Oncol. 29: 358-63.
7.) Bouta EM, McCarthy CW, Keim A, Wang HB, Gilbert RJ, Goldman J. (2011) "Biomaterial guides for lymphatic endothelial cell alignment and migration."?Acta Biomater. 7: 1104-13.?
8.) Hu J, Ye H, Fu A, Chen X, Wang Y, Chen X, Ye X, Xiao W, Duan X, Wei Y, Chen L. (2010) "Deguelin--an inhibitor to tumor lymphangiogenesis and lymphatic metastasis by downregulation of vascular endothelial cell growth factor-D in lung tumor model."?Int J Cancer. 127: 2455-66.
9.) Zhuang Z, Jian P, Longjiang L, Bo H, Wenlin X. (2010) "Altered phenotype of lymphatic endothelial cells induced by highly metastatic OTSCC cells contributed to the lymphatic metastasis of OTSCC cells."?Cancer Sci. 101: 686-92.?
10.) Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ. (2009) "Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model."?Int J Cancer. 124: 2709-18.?
11.) Thapa N, Hong HY, Sangeetha P, Kim IS, Yoo J, Rhee K, Oh GT, Kwon IC, Lee BH. (2008) "Identification of a peptide ligand recognizing dysfunctional endothelial cells for targeting atherosclerosis."?J Control Release. 131: 27-33.?
12.) Yoon CM, Hong BS, Moon HG, Lim S, Suh PG, Kim YK, Chae CB, Gho YS. (2008) "Sphingosine-1-phosphate promotes lymphangiogenesis by stimulating S1P1/Gi/PLC/Ca2+ signaling pathways."?Blood. 112: 1129-38.
13.) Zhuang Z, Jian P, Longjiang L, Bo H, Hongwei Z. (2008) "Identification of oral cancer cell-induced changes in gene expression profile of lymphatic endothelial cell."Cancer Invest. 26: 1002-7.
Question:
Can you tell me the FVIII antibody used to test these cells. What company and catalogue # please.
Answers:
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Posted by Karishma Shah on Tuesday, October 18, 2016
Dear Colleen,
We use Anti-von Willebrand factor antibody (otherwise known as Factor
VIII related antigen) from Sigma-Aldrich. The catalog number is F3520.
Question:
Do these express podoplanin
Answers:
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Posted by Manoj Sharma on Thursday, January 14, 2016
Our quality control department does not test for podoplanin
expression in Human Lymphatic Endothelial Cells. We do test for
endothelial cell markers CD-31 and Factor VIII.
