视网膜是位于眼睛后部的多层结构。它包括感光细胞和视网膜色素上皮细胞（RPEpiC）。RPEpiC位于神经视网膜和脉络膜之间，形成视网膜外屏障，控制着视网膜下腔的化学构成。人们发现RPEpiC内有许多特定偏振分布的细胞内蛋白质。与其他上皮细胞通常顶生面没有基质相比，RPEpiC的顶生面胞膜是与细胞外基质相接触的。RPEpiC在调节相邻的感光膜周转、维生素A的代谢、保护细胞内黑色素所致的视网膜光损害中起关键作用。RPEpiC与很多视网膜疾病的发病相关，如增殖性视网膜病变和年龄相关性黄斑变性。
Sciencell研究实验室的HRPEpiC是从人视网膜分离得到的，原代冻存，冰冻运输。每管细胞密度超过5×10^5/ml。细胞特性经cytokeratine -18, -19和纤维粘连蛋白免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此细胞10倍增增殖。
推荐培养基：上皮细胞培养基 (EpiCM, Cat. #4101)
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货号
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6540
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产地
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美国
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缩写
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HRPEpiC
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规格
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5 x 10^5 /1ml
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用途
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科研
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储存
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液氮
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运输
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干冰
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关键字
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人视网膜色素上皮细胞 HRPEpiC 人视网膜色素上皮细胞 HRPEpiC 人视网膜色素上皮细胞 HRPEpiC 人视网膜色素上皮细胞 HRPEpiC 人视网膜色素上皮细胞 HRPEpiC 人视网膜色素上皮细胞 HRPEpiC 人视网膜色素上皮细胞 HRPEpiC 人视网膜色素上皮细胞 HRPEpiC
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公司简介
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合肥星肽生物科技有限公司作为专业的ScienCell中国代理商，专业经营实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务，对购买项目的前期资料提供,中期合同保证,后期货物跟踪到 *终售后的确保项目准确到位，都有相关专业人士进行维护,确保您在合肥合肥星肽生物科技有限公司公司获得*上等服务！
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联系方式
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何经理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898，
E-mail：info@qingbio.com http://www.qingbio.com QQ:514713116
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1.) Favret S, Binet F, Lapalme E, Leboeuf D, Carbadillo J, Rubic T, Picard E, Mawambo G, Tetreault N, Jean- Joyal S, Chemtob S, Sennlaub F, SanGiovanni JP, Guimond M,Sapieha P. (2013) "Deficiency in the metabolite receptor SUCNR1 (GPR91) leads to outer retinal lesions." Aging (Albany NY). 5: 427.
2.) Miyamoto N, Izumi H, Miyamoto R, Bin H, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Transcriptional Regulation of Activating Transcription Factor 4 under Oxidative Stress in Retinal Pigment Epithelial ARPE-19/HPV-16 Cells." Invest Ophthalmol Vis Sci. 52: 1226-34.
3.) Narayan S, Prasanna G, Tchedre K, Krishnamoorthy R, Yorio T. (2010) "Thrombin-induced endothelin-1 synthesis and secretion in retinal pigment epithelial cells is rho kinase dependent." J Ocul Pharmacol Ther. 26: 389-97.
4.) Bertram KM, Baglole CJ, Phipps RP, Libby RT. (2009) "Molecular regulation of cigarette smoke induced-oxidative stress in human retinal pigment epithelial cells: implications for age-related macular degeneration." Am J Physiol Cell Physiol. 297: C1200-10.
5.) Mannermaa E, Vellonen KS, Ryh?nen T, Kokkonen K, Ranta VP, Kaarniranta K, Urtti A. (2009) "Efflux protein expression in human retinal pigment epithelium cell lines."Pharm Res. 26: 1785-91.
6.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
7.) Swaney JS, Moreno KM, Gentile AM, Sabbadini RA, Stoller GL. (2008) "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res. 87: 367-75.
8.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
Question:
Regarding Human Retinal Pigment Epithelial Cells Catalog #6540 Are the cells from a single human donor or multiple in a pool? If single donor, can one order vials from a few donors to compare? Can you provide a list of available donors and their characteristics? I have read the following paper where this product is used and they make the comment that they conducted their work with hRPEpC in multiple donors. Toxicol Sci. 2015 Mar;144(1):163-72. Retinal Toxicity, in vivo and in vitro, Associated with Inhibition of Nicotinamide Phosphoribosyltransferase.
Answers:
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Hi Elizabeth,
it is Single donor.if you are interested in multiple vials from different lots to get different donors. Please contact us at techsupport@sciencellonline.com to obtain donor information.
Thanks for your interest in our products.
SCIENCELL TEAM
