The doubling time is around 25 hours at early passage and around 40-50 hours at late passage. Cells can undergo 3-4 passages but we highly recommend that customers use the cells at early passage for experiment, as primary cells may change morphology and become senescent over time if not handled properly during subculture.
you can check publication on the next tab or as follows:
1.) Yamanouchi K, Tsuruga E, Oka K, Sawa Y, Ishikawa H. (2012) "Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro." Connect tissue res.53: 14-20.
2.) Kalariya NM, Shoeb M, Reddy AB, Zhang M, van Kuijk FJ, Ramana KV. (2010) "Prevention of endotoxin-induced uveitis in rats by plant sterol guggulsterone." Invest Ophthalmol Vis Sci. 51: 5105-13.
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