人脐静脉内皮细胞 HUVEC

血管内皮细胞有维持血管内环境平衡的功能，血管内皮细胞产生、分泌纤维蛋白凝集和分解系统的活化剂和抑制剂。另外它们调控血小板的粘附和聚集。内皮细胞同时释放分子，调控细胞增殖，控制血管壁功能。人脐静脉内皮细胞是应用*普遍的进行体外研究内皮细胞的细胞类型。脐静脉内皮细胞显微镜下形态如同鹅卵石，用vWF/Factor VIII 和 CD-31容易染色,可以摄入乙酰基化低密度脂蛋白。用IL-1或TNF-α预处理后，可选择性地表达E-selectin和VCAM。
Sciencell研究实验室的脐静脉内皮细胞是从人脐带组织分离得到的，原代冻存，冰冻运输。每管细胞密度超过5×10^5/ml。细胞特性经vWF/Factor VIII和CD31 (P-CAM) 免疫荧光鉴定，能够摄取DiI标记乙酰化低密度脂蛋白(DiI-Ac-LDL)。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此细胞15倍增增殖。

推荐培养基：内皮细胞培养基(ECM, Cat. #1001)

货号	8000
产地	美国
缩写	HUVEC
规格	5 x 10^5 /1ml
用途	科研
储存	液氮
运输	干冰
关键字	人脐静脉内皮细胞 HUVEC   人脐静脉内皮细胞 HUVEC  人脐静脉内皮细胞 HUVEC   人脐静脉内皮细胞 HUVEC  人脐静脉内皮细胞 HUVEC   人脐静脉内皮细胞 HUVEC  人脐静脉内皮细胞 HUVEC   人脐静脉内皮细胞 HUVEC
公司简介	合肥星肽生物科技有限公司作为专业的ScienCell中国代理商，专业经营实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务，对购买项目的前期资料提供,中期合同保证,后期货物跟踪到 *终售后的确保项目准确到位，都有相关专业人士进行维护,确保您在合肥合肥星肽生物科技有限公司公司获得*上等服务！
联系方式	何经理，合肥星肽生物科技有限公司，Tel：0551-63802898      400-8702-898，                                                  E-mail：info@qingbio.com      http://www.qingbio.com      QQ:514713116

1.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242

2.) Yu Y, Yang P, Chuah Q, Huang Y, Peng C, Lee Y, Chiu S. (2013) "Radiation-induced senescence in securin-deficient cancer cells promotes cell invasion involving the IL-6/STAT3 and PDGF-BB/PDGFR pathways."?Sci Rep. 3: 1675

3.) Chen G, Zhao L, Feng J, You G, Sun Q, Li P, Han D, Zhou H. (2013) "Validation of Reliable Reference Genes for Real-Time PCR in Human Umbilical Vein Endothelial Cells on Substrates with Different Stiffness."?PLoS One. 8: e67360

4.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice"?BMC Complement Altern Med. 13: 168.?

5.) Pan Q, Pan H, Lou H, Xu Y, Tian L. (2013) "Inhibition of the angiogenesis and growth of Aloin in human colorectal cancer in vitro and in vivo."?Cancer Cell Int. 13: 69.

6.) Cheng HS, Sivachandran N, Lau A, Boudreau E, Zhao JL, Baltimore D, Delgado-Olguin P, Cybulsky MI, Fish JE. (2013) "MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways."?EMBO Mol Med. 5: 1017-34.?

7.) Huiyong Z, Yong L, Didier M, Yu Z, Jing F, Rongyue C, Jingjing L. (2011) "Enhanced inhibition of murine prostatic carcinoma growth by immunization with or administration of viable human umbilical vein endothelial cells and CRM197."?Braz J Med Biol Res. 44: 140-8.

8.) Zhao Y, Yu L, Xu S, Qiu F, Fan Y, Fu G. (2011) "Down-regulation of connexin43 gap junction by serum deprivation in human endothelial cells was improved by (-)-Epigallocatechin gallate via ERK MAP kinase pathway."?Biochem Biophys Res Commun. 404: 217-22.

9.) Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N. (2010) "Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow."?Epilepsia. 51: 1408-17.

10.) Fang W, Li H, Zhou L, Su L, Liang Y, Mu Y. (2010) "Effect of prostaglandin E1 on TNF-induced vascular inflammation in human umbilical vein endothelial cells."?Can J Physiol Pharmacol. 88: 576-83.

11.) Gao ZX, Huang DY, Li HX, Zhang LN, Lv YH, Cui HD, Zheng JH. (2010) "Scutellarin promotes in vitro angiogenesis in human umbilical vein endothelial cells."?Biochem Biophys Res Commun. 400: 151-6.

12.) Ou Y, Guo XL, Zhai L, Liu XY, Cheng YN. (2010) "TMPDP, a tetramethylpyrazine derivative, protects vascular endothelial cells from oxidation damage by hydrogen peroxide."?Pharmazie. 65: 755-9.

13.) Austin SA, Sens MA, Combs CK. (2009) "Amyloid precursor protein mediates a tyrosine kinase-dependent activation response in endothelial cells."?J Neurosci. 29: 14451-62.

14.) Cueni LN, Detmar M. (2009) "Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions."?Exp Cell Res. 315: 1715-23.?

15.) Li Y, Song YH, Li F, Yang T, Lu YW, Geng YJ. (2009) "MicroRNA-221 regulates high glucose-induced endothelial dysfunction."?Biochem Biophys Res Commun. 381: 81-3.

16.) Vigl B, Zgraggen C, Rehman N, Banziger-Tobler NE, Detmar M, Halin C. (2009) "Coxsackie- and adenovirus receptor (CAR) is expressed in lymphatic vessels in human skin and affects lymphatic endothelial cell function in vitro."?Exp Cell Res. 315: 336-47.

17.) Xiong YQ, Sun HC, Zhang W, Zhu XD, Zhuang PY, Zhang JB, Wang L, Wu WZ, Qin LX, Tang ZY. (2009) "Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells."?Clin Cancer Res. 15: 4838-46.?

18.) Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D. (2008) "Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies."?J Cereb Blood Flow Metab. 28: 312-28.

19.) Yin W, Ghebrehiwet B, Weksler B, Peerschke EI. (2008) "Regulated complement deposition on the surface of human endothelial cells: effect of tobacco smoke and shear stress."?Thromb Res. 122: 221-8.

20.) Gupta GP, Perk J, Acharyya S, de Candia P, Mittal V, Todorova-Manova K, Gerald WL, Brogi E, Benezra R, Massagu? J. (2007) "ID genes mediate tumor reinitiation during breast cancer lung metastasis."?Proc Natl Acad Sci USA. 104: 19506-11.

21.) Ng ES, Cheng ZJ, Ellis A, Ding H, Jiang Y, Li Y, Hollenberg MD, Triggle CR. (2007) "Nitrosothiol stores in vascular tissue: Modulation by ultraviolet light, acetylcholine and ionomycin."?Eur J Pharmacol. 560: 183-92.?

22.) Yin W, Ghebrehiwet B, Weksler B, Peerschke EI. (2006) "Classical pathway complement activation on human endothelial cells."?Mol Immunol. 44: 2228-34.

23.) Ray RB, Basu A, Steele R, Beyene A, McHowat J, Meyer K, Ghosh AK, Ray R. (2004) "Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells."?Virology. 321: 181-8.

24.) D?m?t?r E, Benzakour O, Griffin JH, Yule D, Fukudome K, Zlokovic BV. (2003) "Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1."?Blood. 101: 4797-801.

Question:

Greetings! I am the biosafety officer at our institution, and we've had a researcher order primary HUVEC cells from your company. Would you be able to let us know if you test the cultures/isolates for infectious agents or viruses and what agents you test for?

Answers:

Each cell strain tests negative for HIV, HBV and HCV. However, keep in mind that diagnostic tests are not necessarily 100% accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.