TrkA Stable T17 Cell Line

	Print Version
BioSafety Level	II
Organism	Mouse
SourceOrgan	Brain
Growth Properties	Adherent
Morphology	Polygonal
Description	TrkA Stable T17 Cell Line expresses rat TrkA (Tropomyosin receptor kinase A) after TrkA plasmid was successfully transfected into SN56 cells and were selected in the presence of G418. TrkA is the receptor for neurotrophins, such as nerve growth factor (NGF), which plays essential role in the nervous system – in particular apoptotic cell death prevention, and/or the promotion of cellular differentiation and axon elongation in neurons. Researchers may use the TrkA Stable T17 Cell Line to screen for NGF-like compounds that would act as TrkA agonists to study the mechanisms of the signaling pathways and affects NGF. The lack of TrkA has been associated with the neurodegenerative processes and thus the cell line is a valuable tool to conduct related studies. abm has available the TrkB Stable T48 Cell Line (T3159), which can be used to screen BDNF-like (brain-derived neurotrophic factor –like) compounds as well.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Over Expression	Rat TrkA receptor via transfection and G418 antibiotic selection
Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium to final concentration of 10% fetal bovine serum (TM999), 1 mM Sodium Pyruvate Solution(TM057), 1% Penicillin/Streptomycin (G255), and 300 mg/ml G418 (G271). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
Pictures
Quality Control	Selection with 300 mg/ml G418
References	Jang, S., Okada, M., Sayeed, I., Xiao, G., Stein, D., Jin, P., & Ye, K. (2007). Gambogic amide, a selective agonist for TrkA receptor that possesses robust neurotrophic activity, prevents neuronal cell death. Proceedings of the National Academy of Sciences, 104(41), 16329-16334. doi:10.1073/pnas.0706662104
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
关键字	TrkA Stable T17 Cell Line；TrkA Stable T17 Cell Line；TrkA Stable T17 Cell Line；TrkA Stable T17 Cell Line；TrkA Stable T17 Cell Line；TrkA Stable T17 Cell Line
公司简介	合肥星肽生物科技有限公司作为专业的ABM中国代理商，专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。ABM安徽代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务，对购买项目的前期资料提供,中期合同保证,后期货物跟踪到*终售后的确保项目准确到位，都有相关专业人士进行维护,确保您在合肥合肥星肽生物科技有限公司公司获得*上等服务！
联系方式	何经理，合肥星肽生物科技有限公司，Tel：0551-63802898      400-8702-898，                                                  E-mail：info@qingbio.com      http://www.qingbio.com      QQ:514713116

选择合肥星肽生物科技有限公司的理由

生产实力雄厚

合肥星肽生物科技有限公司拥有近2000平方米独立研发中心以及无菌实验室4条完善的多肽生产线，单月可交货3000条多肽精品，生产研发速度快，可快速相应您的需求。

技术设备先进

合肥星肽生物科技有限公司实验室配备了waters LC-MA/MS、waters uplc，waters全自动纯化仪、岛津LCMS、全自动冻干系统、紫外分光光度计等专用精密仪器，为您提供高效专业的技术服务。

客户服务完善

合肥星肽生物科技有限公司广大客户提供专业的前沿产品、合理的价格、便捷的物流服务，帮助客户缩短科研周期、节省试验成本，使客户的科学研究具有持续性。

关于合肥星肽生物科技有限公司

合肥星肽生物科技有限公司是一家致力于生命科学和生物技术领域的高科技企业，公司地址位于科教名城———合肥市。公司主营多肽合成、多肽定制、多肽纯化等多肽制备多肽技术服务。在合肥市与中科院的关怀下，2021年初合肥市高新区与中科院分别与合肥星肽生物科技有限公司签订了战略投资协议。 公司位于合肥市高新区安徽中科资城创业孵化器A区，拥有2000平方米独立研发中心以及无菌实验室，四条完善的多肽生产线，配备了Waters LC- MS/MS、Waters UPLC、Waters 全自动纯化仪、岛津LCMS、全自动冻干系统、紫外分光光度计等专用精密仪器保障合肥星肽生物科技有限公司月产3000条精品多肽的生产能力，这些得以让我们可以为客户快速准确的提供各种高质量的多肽产品及服务。 合肥合肥星肽生物科技有限公司始终致力于为您的实验研究提供高品质、稳定性良好的产品，“做您身边的多肽合成专家”一直是每个合肥星肽生物科技有限公司人的目标与方向，励志作为多肽合成行业标准的风向标。公司拥有100余人的专业团队，其中博士研究生、硕士研究生10余人。在此基础上公司配备了一套包含从售前到售后的专业团队，力争做到每个环节都“清晰明了”，对每一个问题都“吹毛求疵”，让您的实验变得更加简单，正是凭借这样的信仰和扎实的多肽合成技术优势，合肥星肽生物科技有限公司已经成为国内外高校与众多生物医药企业诚信的合作伙伴和坚强的技术后盾。 公司秉承“专业锻造品质，服务打造市场”的经营理念，为国内广大客户提供专业的前沿产品、合理的价格、便捷的物流服务。 公司自成立以来，一直致力于多肽合成、多肽定制、多肽纯化等多肽制备多肽技术服务的研究。帮助客户缩短科研周期、节省试验成本，使客户的科学研究具有持续性。 实验室配备一支年轻、专业、富有创造精神且经验丰富的技术团队。 合肥星肽生物科技有限公司目前跟国内外企业有着友好的合作往来。斯坦福大学，牛津大学，北京大学，清华大学，天津大学，浙江大学，中科大，香港理工大学，以及中科院遗传所，过程所，化学所，生物细胞所，微生物所等，北京军科院**医学研究所，武汉病毒研究所等有稳定合作。

订购信息

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!