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Print Version
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BioSafety Level
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II C2C12 Cells
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Organism
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Mouse
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Source Organ
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Myoblast C2C12 Cells C2C12 Cells
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Growth Properties
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Adherent
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Morphology
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Elongated
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Recommended Seeding Density
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Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
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Applications
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For Research Use Only C2C12 Cells C2C12 Cells
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Description
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C2C12 Cells (YAP1-GFP11 Clone) was established through transfection of retrovirus vector to carry the eleventh β-strand of GFP fused to the c-terminus of FLAG-14-3-3 fused with YAP1. YAP1 promotes cell cycle of cells and suppresses myogenesis. It can be used as a research tool to study affects on myogenesis suppression and its effects.
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Procedure Overview
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Propagation
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The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
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Reference
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Kodaka, M., Yang, Z., Nakagawa, K., Maruyama, J., Xu, X., Sarkar, A., . . . Hata, Y. (2015). A new cell-based assay to evaluate myogenesis in mouse myoblast C2C12 cells. Experimental Cell Research, 336(2), 171-181. doi:10.1016/j.yexcr.2015.06.015
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Disclaimer
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1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
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关键词
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C2C12 Cells (YAP1-GFP11 Clone)；C2C12 Cells (YAP1-GFP11 Clone)；C2C12 Cells (YAP1-GFP11 Clone)；C2C12 Cells (YAP1-GFP11 Clone)；C2C12 Cells (YAP1-GFP11 Clone)；C2C12 Cells (YAP1-GFP11 Clone)
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公司简介
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合肥星肽生物科技有限公司作为专业的ABM中国代理商，专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。ABM安徽代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务，对购买项目的前期资料提供,中期合同保证,后期货物跟踪到*终售后的确保项目准确到位，都有相关专业人士进行维护,确保您在合肥合肥星肽生物科技有限公司公司获得*上等服务！
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联系方式
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何经理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898，
E-mail：info@qingbio.com http://www.qingbio.com QQ:514713116
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